Incorrect pipetting is one of the most common sources of error in enzymatic wine testing.
If you're new to laboratory equipment, understanding how to properly use your pipettes is essential for accurate, reliable results with Absorbance One and Vintessential™ test kits.
In this video, we cover:
✔ The three pipettes supplied (100–1000 µL, 10–100 µL, 2–20 µL)
✔ Understanding microlitres (µL) vs millilitres (mL)
✔ Setting the correct volume
✔ First stop vs second stop technique
✔ How to aspirate and dispense accurately
✔ Why you must change tips between reagents
✔ Maintaining calibration and accuracy
Each Vintessential™ protocol is written in microlitres, so correct volume selection and technique are critical.
✔ Good pipetting technique directly impacts analytical accuracy and repeatability.
Remember:
✔ Press to the first stop before drawing liquid
✔ Dispense to the second stop to expel the full volume
✔ Never reuse tips between reagents
✔ Keep pipettes clean and protected
If pipettes are dropped, contaminated or poorly maintained, they may lose accuracy and require recalibration.
“Good technique = reliable results.”
