Amino Acid | Test kit for Autoanalyser

During fermentation of grape juice, yeasts require nitrogen as a nutrient, with a portion provided by primary amino acids. This test kit is suitable for measuring the Primary Amino Acid Nitrogen (PAAN) content in grape juice and non-fermenting must.

The Yeast Assimilable Nitrogen (YAN) content of the juice can be determined by adding this PAAN content to the Ammonia Nitrogen (AN) content.

Analytical principle

The Vintessential™ Amino Acid test kit provides automated determination of primary amino nitrogen concentrations in juice, must and wine samples. The method is ideally suited to laboratories seeking efficient monitoring of fermentation nutrients across multiple samples.

Primary amino acids react with ortho-phthaldialdehyde (OPA) and N-acetyl-L-cysteine (NAC) to form a coloured isoindole complex. The resulting absorbance is measured automatically and is directly proportional to the concentration of primary amino nitrogen present in the sample.

Key parameters

Analyte: Primary Amino Nitrogen (PAN)

Detection Method: OPA/NAC Colourimetric Analysis

Sample Type: Grape Juice, Must and Wine

Typical uses

• Yeast Assimilable Nitrogen (YAN) programs
• Fermentation nutrient management
• Juice and must assessment
• Harvest intake testing
• Fermentation optimisation
• Routine laboratory quality assurance

Resources

Instructions:
4B110 PAAN Autoanalyser InsertIssued 27082025

Material Safety Data Sheets:

PAAN DA Vial 1 (Buffer)
PAAN DA Vial 2 (NAC)
PAAN DA Vial 3 (OPA)