Ammonia | Test kit for Autoanalyser

Ammonia is found in both grape juice and wine. It is an important nutrient for yeast during primary fermentation.

The Yeast Assimilable Nitrogen (YAN) content of the juice can be determined by adding the Ammonia Nitrogen (AN) content to the Primary Amino Acid Nitrogen (PAAN) content.

Analytical principle

The Vintessential™ Ammonia Test Kit enables automated measurement of ammonia concentrations in juice, must and wine samples. The assay supports routine fermentation monitoring by providing rapid and reliable analysis of an important yeast nutrient.

Ammonium ions react with α-ketoglutarate and NADH in the presence of glutamate dehydrogenase (GlDH) to form L-glutamate. The reduction in NADH concentration is measured automatically and is directly proportional to the ammonia concentration present in the sample.

Key parameters

Analyte: Ammonia

Measurement Wavelength: 340 nm

Detection Method: Enzymatic (NADH)

Sample Type: Juice, Must and Wine

Typical uses

• Nitrogen management programs
• Fermentation monitoring
• Yeast nutrition assessment
• Juice and must analysis
• Fermentation troubleshooting
• Routine quality control testing

Resources

Instructions:
4B120 Ammonia Autoanalyser Insert

Material Safety Data Sheets:

Ammonia DA Vial 1 (Buffer)
Ammonia DA Vial 2 (NADH)
Ammonia DA Vial 3 (GlDH)