Ammonia | Test kit for Spectrophotometer

Ammonia is found in both grape juice and wine. It is an important nutrient for yeast during primary fermentation.

The amount of NADH consumed is measured at 340 nm and is stoichiometrically related to the amount of ammonia present. The Yeast Available Nitrogen (YAN) content of the juice can be determined by adding the Ammonia Nitrogen (AN) content to the Primary Amino Acid Nitrogen (PAAN) content.

Analytical principle

The Vintessential™ Ammonia Test Kit utilises an enzymatic reaction involving glutamate dehydrogenase (GlDH) to determine ammonia concentrations in wine and must samples.

Ammonium ions react with α-ketoglutarate and NADH to form L-glutamate. The decrease in NADH concentration is measured spectrophotometrically at 340 nm and is directly proportional to the ammonia concentration present in the sample.

This method provides reliable analysis for monitoring fermentation nutrients and nitrogen management.

Key parameters

Analyte: Ammonia

Measurement Wavelength: 340 nm

Operating Temperature: 20–25°C

Blank / Zero Setting: Against air without a cuvette in the light path

Resources

Instructions:
4A120 Ammonia 30

Material Safety Data Sheets:

Ammonia Vial 1 (Buffer)
Ammonia Vial 2 (NADH)
Ammonia Vial 3 (GlDH)
Ammonia Vial 4 (Standard)