Glucose/Fructose | Test kit for Autoanalyser

This kit has been designed to work perfectly with our new Chemwell Discrete Analysers (and Thermo’s Arena and Gallery Analysers). All the volumes are set to run out at the same time, so you won’t suffer any wastage.

As the main sugars found in grape juice and wine, glucose and fructose can be determined enzymatically by using this spectrophotometric assay.

Analytical principle

The Vintessential™ Glucose / Fructose test kit provides automated analysis of fermentable sugars throughout fermentation and finished wine production. Automated workflows enable efficient processing of multiple samples while maintaining analytical consistency.

Glucose and fructose are quantified through a sequence of enzymatic reactions involving hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PDH) and phosphoglucose isomerase (PGI). The formation of NADPH is measured automatically at 340 nm and is directly proportional to the concentration of glucose and fructose present in the sample.

Key parameters

Analyte: Glucose and Fructose

Measurement Wavelength: 340 nm

Detection Method: Enzymatic (NADPH)

Sample Type: Juice, Must and Wine

Typical uses

• Residual sugar analysis
• Fermentation monitoring
• Fermentation completion verification
• Sweet wine production
• Bottling quality assurance
• Routine laboratory testing

Resources

Instructions:
4B140 D Glucose D Fructose Autoanalyser Insert

Material Safety Data Sheets:

Glucose-Fructose DA Vial 1 (Buffer-Coenzymes)
Glucose-Fructose DA Vial 2 (HK-G6PDH)
Glucose-Fructose DA Vial 3 (PGI)