L-Malic Acid | Test kit for Autoanalyser

This kit has been designed to work perfectly with our new Chemwell Discrete Analysers (and Thermo’s Arena and Gallery Analysers). All the volumes are set to run out at the same time, so you won’t suffer any wastage.

Malo-lactic fermentation occurs in wine when MLF bacteria convert malic acid to lactic acid. This spectrophotometric assay accurately determines the levels of malic acid, allowing MLF progress to be monitored and ensuring that conversion is complete.

Analytical principle

The Vintessential™ L-Malic Acid Test Kit enables automated monitoring of malic acid concentrations throughout fermentation and maturation. The assay is particularly valuable for tracking malolactic fermentation progress and confirming completion.

L-malic acid is converted through enzymatic reactions involving malate dehydrogenase (MDH) and glutamate oxaloacetate transaminase (GOT), generating NADH which is measured automatically. The absorbance recorded is directly proportional to the concentration of malic acid present in the sample.

Key parameters

Analyte: L-Malic Acid

Measurement Wavelength: 340 nm

Detection Method: Enzymatic (NADH)

Sample Type: Juice, Must and Wine

Typical uses

• Malolactic fermentation monitoring
• Fermentation completion verification
• Grape maturity assessment
• Production quality control
• Wine stability programs
• Routine laboratory analysis

Resources

Instructions:
4B160 L Malic Acid Autoanalyser Insert

Material Safety Data Sheets:

Malic Acid DA Vial 1 (Buffer)
Malic Acid DA Vial 2 (NAD)
Malic Acid DA Vial 3 (GOT-MDH)