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Total SO₂ | Test kit for Spectrophotometer

Sulfur dioxide is used as a preservative in wine, and there are restrictions limiting the amount that can be added in most wine-producing countries. This test kit is a fast and easy way to determine the amount of total sulfur dioxide in wine samples, without the need for the laborious setup associated with traditional methods.

Additionally, unlike some other commercial products, this test kit does not contain formaldehyde, which is a known carcinogen.

For this Test Kit, semi-micro cuvettes are required.

Principle of measurement

The amount of sulfite present in wine is measured by monitoring the reaction with a chromogen under basic conditions. The reduction of the chromogen leads to formation of a strongly absorbing compound which can be measured at 340 nm. The measured amount of the activated chromogen is stoichiometrically proportional to the amount of total sulphite present.

Analytical principle

The Vintessential™ Total SO₂ Test Kit utilises a colourimetric method to determine the concentration of total sulfur dioxide present in wine samples.

Sulfite ions (SO₃²⁻) react with a reduced chromogen to produce an activated blue-coloured compound. The intensity of the colour formed is measured spectrophotometrically at 340 nm and is directly proportional to the concentration of total sulfur dioxide in the sample.

This method provides accurate and repeatable results suitable for routine wine analysis and quality control applications.

Key parameters

Analyte: Total Sulfur Dioxide (SO₂)

Measurement Wavelength: 340 nm

Cuvette Requirements: Semi-micro cuvettes with 1 cm path length

Operating Temperature: 20–25°C

Final Volume in Cuvette: 1.48 mL

Blank / Zero Setting: Against air without a cuvette in the light path

Suitable applications

  • Routine winery laboratory analysis
  • Sulfur dioxide management programs
  • Wine quality assurance
  • Production and storage monitoring
  • Regulatory and compliance testing

Resources

Instructions:
4A200 Total SO2 30 test

Material Safety Data Sheets:

Total SO2 Vial 1 (Buffer)
Total SO2 Vial 2 (Chromogen)
Total SO2 Vial 3 (Standard)

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